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Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios
Yik Sung, Y.; Van Damme, E.J.M.; Sorgeloos, P.; Bossier, P. (2007). Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios. Fish Shellfish Immunol. 22(4): 318-326. dx.doi.org/10.1016/j.fsi.2006.05.008
In: Fish & Shellfish Immunology. Academic Press: London; New York. ISSN 1050-4648; e-ISSN 1095-9947, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Biological phenomena > Biosynthesis
    Biology > Microbiology
    Biology > Physiology
    Developmental stages > Larvae
    Environmental effects > Temperature effects > Heat shock
    Heat shock proteins
    Immune response
    Pathogens
    Peptides > Proteins > Heat shock proteins
    Survival
    Virulence
    Artemia franciscana Kellog, 1906 [WoRMS]; Vibrio campbellii (Baumann, Baumann & Mandel, 1971) Baumann, Baumann, Bang & Woolkalis, 1981 [WoRMS]; Vibrio proteolyticus (Merkel et al., 1964) Baumann et al., 1982 [WoRMS]
    Marien/Kust
Author keywords
    non-lethal heat-shock; heat shock proteins; Artemia franciscana; immune response; challenge test; Vibrio campbellii; Vibrio proteolyticus
Auteurs  Top 
  • Yik Sung, Y., meer
  • Van Damme, E.J.M., meer
  • Sorgeloos, P., meer
  • Bossier, P., meer
Abstract
    Brine shrimp Artemia were exposed under gnotobiotic conditions to a non-lethal heat shock (NLHS) from 28 to 32, 37 and 40°C. Different recovery periods (2, 6, 12 and 24 h) and different heat-exposure times (15, 30, 45 and 60 min) were tested. After these NLHS, Artemia was subsequently challenged with Vibrio. Challenge tests were performed in stressed and unstressed nauplii at concentrations of 107 cells ml-1 of pathogenic bacteria, Vibrio campbellii and Vibrio proteolyticus. A NLHS with an optimal treatment of 37°C for 30 min and a subsequent 6 h recovery period resulted in a cross-protection against pathogenic Vibrio. A 100% increase in the larval survival (P < 0.05) was observed. We have also demonstrated by Western blot that a NLHS increases the expression of HSP-70 in heat-shocked (HS) treated animals. This report is the first to reveal a cross protection of a NLHS against deleterious bacterial challenges in living crustaceans. The putative role of heat shock proteins (HSPs) in this process is discussed.
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