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Contamination of cold-smoked fish with Listeria monocytogenes
Aalberts, C.; van Pelt, H.; Luten, J. (2000). Contamination of cold-smoked fish with Listeria monocytogenes, in: Georgakis, S.A. (Ed.) Proceedings of 29th WEFTA meeting 10-14 october, 1999 - Leptocarya - Pieria, Greece. pp. 60-68
In: Georgakis, S.A. (Ed.) (2000). Proceedings of 29th WEFTA meeting 10-14 october, 1999 - Leptocarya - Pieria, Greece. Greek Society of Food Hygienists and Technologists: Thessaloniki. , meer
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  • Aalberts, C.
  • van Pelt, H.
  • Luten, J., meer
Abstract
    In the Netherlands, 41% of ready-to-eat cold-smoked fish is contaminated with the human pathogenic bacterium Listeria monocytogenes. Therefore sources of contamination in fish smoking plants were investigated. A survey in three plants shows that both L. monocytogenes as L. spp are easy to isolate from floors, machines, tables, and hands and aprons of employees. Total elimination of contamination will not be possible, so the focus will be on measures to control contamination like the use of risk assessment in the evaluation of product safety, improvement of cleaning and disinfection procedures, and monitoring of processing steps. To enable tracing of contamination routes, two methods of typing of L. monocytogenes are set up. A combination of limited serotyping and resistance to tetracycline, cadmium, and arsenic provides a set of 9 biotypes. Analysis of samples from different European countries shows large differences in the presence of the various biotypes. A more discriminatory typing method, AFLP , will be used further in attempt to identify contamination routes.
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