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Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka
Abeysinghe, P.D.; Triest, L.; De Greef, B.; Koedam, N.; Hettiarachi, S. (1999). Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka. Hydrobiologia 413: 11-16. https://dx.doi.org/10.1023/A:1003899028558
In: Hydrobiologia. Springer: The Hague. ISSN 0018-8158; e-ISSN 1573-5117, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Biology > Genetics > Population genetics
    Distribution > Geographical distribution
    Genetic parameters > Genetic variance
    Markers > Genetic markers > Dna > Random amplified polymorphic dna
    Natural populations > Plant populations
    Polymerase chain reaction
    Subpopulations
    Water bodies > Inland waters > Wetlands > Swamps > Mangrove swamps
    Bruguiera gymnorhiza (L.) Lamk. [WoRMS]; Bruguiera sexangula (Lour.) Poir. [WoRMS]
    Marien/Kust; Brak water
Auteurs  Top 
  • Abeysinghe, P.D., meer
  • Triest, L., meer
  • De Greef, B.
  • Koedam, N., meer
  • Hettiarachi, S.
Abstract
    The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened, 20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a small number of primers.
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