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The application of microarray technology to the identification of Tc1-like element sequences in fish genomes
Wenne, R.; Handschuh, L.; Pocwierz-Kotus, A.; Urbaniak, R.; Formanowicz, P.; Calkiewicz, J.; Brzozowska, K.; Figlerowicz, M.; Wegrzyn, G.; Wröbel, B. (2011). The application of microarray technology to the identification of Tc1-like element sequences in fish genomes. Mar. Biol. Res. 7(5): 466-477. https://dx.doi.org/10.1080/17451000.2010.527990
In: Marine Biology Research. Taylor & Francis: Oslo; Basingstoke. ISSN 1745-1000; e-ISSN 1745-1019, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Acids > Organic compounds > Organic acids > Nucleic acids > DNA
    Fauna > Aquatic organisms > Aquatic animals > Fish
    Genomes
    Transposons
    Marien/Kust
Author keywords
    DNA microarray; fish; genome; Tc1; transposon
Auteurs  Top 
  • Wenne, R., meer
  • Handschuh, L.
  • Pocwierz-Kotus, A.
  • Urbaniak, R.
  • Formanowicz, P.
  • Calkiewicz, J.
  • Brzozowska, K.
  • Figlerowicz, M.
  • Wegrzyn, G.
  • Wröbel, B.
Abstract
    The phylogenetic relationships between Tc1 transposable elements have previously been reported for the genomes of some fish species. However, research in this field has been hindered by the low number of fish genome sequences available in databases. The application of a DNA microarray as a universal tool for Tc1 transposon sequence analysis in fish genomes is described here. A prototype oligonucleotide microarray was constructed and used to compare samples of genomic DNA isolated from selected fish species. These results, combined with earlier reported molecular analysis of Tc1 showed the usefulness of DNA microarray in the screening of transposon sequences.
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