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Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius
Nedashkovskaya, O.I.; Van Trappen, S.; Frolova, G.M.; De Vos, P. (2012). Bacillus berkeleyi sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Arch. Microbiol. 194(3): 215-221. http://dx.doi.org/10.1007/s00203-011-0771-0
In: Archives of Microbiology. Springer: Heidelberg; Berlin. ISSN 0302-8933; e-ISSN 1432-072X, meer
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| Trefwoorden |
Bacillus berkeleyi
Marien/Kust |
| Author keywords |
Bacillus berkeleyi; Aerobic marine spore-forming bacteria; Phylogeny; Taxonomy |
| Auteurs | | Top |
- Nedashkovskaya, O.I.
- Van Trappen, S., meer
- Frolova, G.M.
- De Vos, P., meer
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| Abstract |
A bacterial strain, designated KMM 6244T, was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile and spore-forming. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bacillus. The nearest neighbor of strain KMM 6244T was Bacillus decolorationis LMG 19507T with a 16S rRNA gene sequence similarity of 98.0%. Sequence similarities with the other recognized Bacillus species were less than 96.0%. The results of the DNA–DNA hybridization experiments revealed a low relatedness (37%) of the novel isolate with the type strain of B. decolorationis LMG 19507T. Strain KMM 6244T grew at 4–45°C and with 0–12% NaCl. It produced catalase and oxidase and hydrolyzed aesculin, casein, gelatin and DNA. The predominant fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, C15:0, iso-C16:0 and iso-C14:0. The DNA G + C content was 39.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6244T represents a novel species in the genus Bacillus, for which the name Bacillus berkeleyi sp. nov. is proposed. The type strain is KMM 6244T (KCTC 12718T = LMG 26357T). |
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