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Proteomic responses of peripheral blood mononuclear cells in the European eel (Anguilla anguilla) after perfluorooctane sulfonate exposure
Roland, K.; Kestemont, P.; Henuset, L.; Pierrard, M.-A.; Raes, M.; Dieu, M.; Silvestre, F. (2013). Proteomic responses of peripheral blood mononuclear cells in the European eel (Anguilla anguilla) after perfluorooctane sulfonate exposure. Aquat. Toxicol. 128-129: 43-52. dx.doi.org/10.1016/j.aquatox.2012.10.016
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514, meer
Peer reviewed article  
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Trefwoord
    Anguilla anguilla (Linnaeus, 1758) [WoRMS]
Author keywords
    Anguilla anguilla; PFOS; PBMC; Proteomics; 2D-DIGE
Auteurs  Top 
  • Roland, K., meer
  • Kestemont, P., meer
  • Henuset, L., meer
  • Pierrard, M.-A.
Abstract
    Since the 1980s, the stocks of European eel have been declining in most of their geographical distribution area. Many factors can be attributed to this decline such as pollution by xenobiotics like perfluorooctane sulfonate (PFOS). This study aimed at evaluating the in vitro toxicity of eel peripheral blood mononuclear cells (PBMC) exposed to PFOS. Exposure time and two concentrations were chosen to avoid cell mortality (48 h exposure at 10 mu g PFOS/L and 1 mg PFOS/L). After in vitro contaminations, the post-nuclear fraction was isolated and a proteomic analysis using 2D-DIGE was performed to compare PBMC from the control group with cells exposed to the pollutant. On the 158 spots that were significantly affected by PFOS exposure, a total of 48 different proteins were identified using nano-LCESI-MS/MS and the Peptide and Protein Prophet of Scaffold software. These proteins can be categorized into diverse functional classes, related to cytoskeleton, protein folding, cell signaling, proteolytic pathway and carbohydrate and energy metabolism, which provide clues on the cellular pathways mainly affected by PFOS. Some of the identified proteins are rarely found in other ecotoxicological proteomic studies and could constitute potential biomarkers of exposure to PFOS in fish.
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