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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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Lactate dehydrogenase isozyme pattern of the Mediterranean killifish Aphanius fasciatus (Teleostei, Cyprinodontidae) Maltagliati, F. (1999). Lactate dehydrogenase isozyme pattern of the Mediterranean killifish Aphanius fasciatus (Teleostei, Cyprinodontidae). Ital. J. Zoolog. 66: 171-174. https://dx.doi.org/10.1080/11250009909356252 In: Italian Journal of Zoology. Taylor & Francis/Mucchi Editore: Modena. ISSN 1125-0003; e-ISSN 1748-5851, meer

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Beschikbaar in	Auteur
VLIZ: Open access 24345 [ download pdf ]

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Trefwoorden

Biological phenomena > Evolution Conservation Enzymes > Isoenzymes Fauna > Aquatic organisms > Aquatic animals > Fish Fauna > Aquatic organisms > Aquatic animals > Fish > Estuarine organisms > Brackishwater fish Isoelectric focusing Techniques > Biological techniques > Electrophoresis > Isoelectric focusing Cyprinodontidae Wagner, 1828 [WoRMS] Mediterranean [Marine Regions] Brak water

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Auteur		Top
Maltagliati, F., meer

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Abstract

Electrophoretic analyses of the tetrameric lactate dehydrogenase isozyme patterns of the endangered cyprinodontid fish Aphanius fasciatus were carried out. Organ and tissue samples of ten individuals of each sex were analysed by cellulose acetate electrophoresis and isoelectric focusing. Isozyme electrophoretic patterns indicated that three LDH loci (Ldh-A, Ldh-B and Ldh-C) were active and showed differential tissue expressions. No differences between sexes were observed. The Ldh-A and Ldh-B loci were expressed in all tissues analysed, and the A subunits possessed a net negative charge greater than B subunits. Heterotetramers including subunit A were not observed, but a complex heterotetramer banding pattern of subunits B and C was detected. On the basis of the staining intensity of the electromorphs, Ldh-A predominated in all tissues analysed, with the exception of the liver, where Ldh-B predominated. Instead, Ldh-C was characterised by high relative anodal mobility and was expressed only in the eye. Furthermore, at this locus two alleles were observed in specimens analysed. The LDH banding pattern of A. fasciatus was consistent with that of advanced teleosts and it showed an inversion of the net negative charge of products of the Ldh-A and Ldk-B loci.

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