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Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi
Van Houdt, J.K.J.; Hellemans, B.; Van de Putte, A.; Koubbi, P.; Volckaert, F.A.M. (2006). Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi. Mol. Ecol. Notes 6(1): 157-159. dx.doi.org/10.1111/j.1471-8286.2005.01174.x
In: Molecular Ecology Notes. Blackwell Publishers: Oxford. ISSN 1471-8278; e-ISSN 1471-8286, meer
Peer reviewed article  
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Trefwoorden
    Nototheniidae Günther, 1861 [WoRMS]; Trematominae Balushkin, 1982 [WoRMS]
    Marien/Kust
Author keywords
    Antarctic; microsatellite; Nototheniidae; nuclear marker; Trematomids
Auteurs  Top 
  • Van Houdt, J.K.J., meer
  • Hellemans, B., meer
  • Van de Putte, A., meer
  • Koubbi, P., meer
  • Volckaert, F.A.M., meer
Abstract
    Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adelie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies.
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