Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints
Ivanova, E.P.; Matte, G.R.; Matte, M.H.; Coenye, T.; Huq, A.; Colwell, R.R. (2002). Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints. Syst. Appl. Microbiol. 25(2): 275-283. https://dx.doi.org/10.1078/0723-2020-00103
In: Systematic and Applied Microbiology. Elsevier: Jena. ISSN 0723-2020; e-ISSN 1618-0984, meer
|  |
| Trefwoorden |
Pseudoalteromonas Gauthier, Gauthier & Christen, 1995 [WoRMS]
Marien/Kust |
| Author keywords |
Pseudoalteromonas; biogeography; REP; ERIC; BOX-PCR |
| Auteurs | | Top |
- Ivanova, E.P.
- Matte, G.R.
- Matte, M.H.
|
- Coenye, T., meer
- Huq, A.
- Colwell, R.R.
|
|
| Abstract |
DNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67–70% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
