Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Phytoplankton Virus Production Negatively Affected by Iron Limitation
Slagter, H.A.; Gerringa, L.J.A.; Brussaard, C.P.D. (2016). Phytoplankton Virus Production Negatively Affected by Iron Limitation. Front. Mar. Sci. 3: 156. dx.doi.org/10.3389/fmars.2016.00156
In: Frontiers in Marine Science. Frontiers Media: Lausanne. e-ISSN 2296-7745, meer
|   |
| Author keywords |
algae; marine phytoplankton; marine viruses; iron; Fe speciation; Fe limitation |
| Auteurs | | Top |
- Slagter, H.A., meer
- Gerringa, L.J.A., meer
- Brussaard, C.P.D., meer
|
|
|
| Abstract |
Fe-limited monocultures of the ubiquitous algae Micromonas pusilla and Phaeocystis globosa were infected with their respective viruses (MpV and PgV) to ascertain the effect of Fe-limitation on phytoplankton host-virus dynamics. The effect of the viral shunt on Fe concentrations and bioavailability is starting to gain attention, since not only is Fe released through lysis, but also its solubility is increased by the simultaneous release of Fe-binding dissolved organic ligands. However, the effect of Fe-limitation on the process of viral lysis itself is poorly understood. In this study fine adjustment of a seawater-based culture medium including the use of ultra-clean trace metal conditions and protocols allowed for Fe-limited growth at nanomolar amounts as opposed to micromolar amounts typically employed in culturing. Viral lysates derived from Fe-limited and Fe-replete (for comparison) hosts were cross-inoculated in hosts of both Fe treatments, to judge the quality of the resulting lysate as well as the effect of Fe introduction after initial infection. For both phytoplankton host-virus systems, the virus burst size reduced strongly under Fe stress, i.e., on average 28 ± 1% of replete. Moreover, the MpV virus progeny showed highly reduced infectivity of 30 ± 7%, whereas PgV infectivity was not affected. A small addition of Fe to Fe-limited cultures coming from the Fe-replete lysate counteracted the negative effect of Fe-limitation on phytoplankton virus production to some extent (but still half of replete), implying that the physiological history of the host at the moment of infection was an important underlying factor. These results indicate that Fe-limitation has the strong potential to reduce the loss of phytoplankton due to virus infection, thereby affecting the extent of Fe-cycling through the viral shunt. To what extent this affects the contribution of viral lysis-induced organic ligand release needs further study. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
