Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles
Sui, L.; Ma, G.; Lu, W.L.; Deng, Y.; Bossier, P.; De Schryver, P. (2016). Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles. Aquac. Res. 47(11): 3644-3652. https://dx.doi.org/10.1111/are.12817
In: Aquaculture Research. Blackwell: Oxford. ISSN 1355-557X; e-ISSN 1365-2109, meer
| |
Trefwoord |
Eriocheir sinensis H. Milne Edwards, 1853 [WoRMS]
|
Author keywords |
poly-beta-hydroxybutyrate (PHB); Eriocheir sinensis (Milne-Edwards);growth; enzyme activity; gut microbiota |
Auteurs | | Top |
|
- Deng, Y.
- Bossier, P., meer
- De Schryver, P., meer
|
|
Abstract |
Poly-β-hydroxybutyrate (PHB) is microbial carbon and energy storage polymer, which can be degraded into water-soluble β-hydroxybutyric acid in the gastrointestinal tract of aquatic animals. A 60-day culture experiment was performed using Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles with an average initial body weight of 0.74 ± 0.06 g which were fed a diet supplemented with 0%, 0.5%, 1%, 3% or 5% PHB. A PHB dietary supplementation of 1% and 3% significantly improved the body weight gain, moulting frequency and concomitantly reduced 2nd–3rd moulting intervals of the crabs (P < 0.05). The dietary PHB level positively related to hepatopancreatic pepsin, trypsin and lipase activity (P < 0.05). Increasing the dietary PHB also improved total superoxide dismutase activity, but reduced alkaline phosphatase and acid phosphatase activity in the serum of hemolymph (P < 0.05). A 16S rRNA gene analysis by denaturing gradient gel electrophoresis indicated that PHB supplementation led to a significantly higher range-weighted richness, diversity and evenness of the gut bacterial community when dosed at 3% in the feed. The beneficial effects of PHB are discussed in terms of immune defense, metabolism and gut microbiota of the crabs. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.