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Discovering novel enzymes by functional screening of plurigenomic libraries from alga-associated Flavobacteriia and Gammaproteobacteria
Martin, M.; Vandermies, M.; Joyeux, C.; Martin, R.; Barbeyron, T.; Michel, G.; Vandenbol, M. (2016). Discovering novel enzymes by functional screening of plurigenomic libraries from alga-associated Flavobacteriia and Gammaproteobacteria. Microbiological Research 186-187: 52-61. https://dx.doi.org/10.1016/j.micres.2016.03.005
In: Microbiological Research: Jena. ISSN 0944-5013, meer
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| Trefwoorden |
Bacteria [WoRMS]
Marien/Kust |
| Author keywords |
Alga-associated microflora; Marine bacteria; Agarase; lota-carrageenase;Kappa-carrageenase; Esterase; Beta-glucosidase; Xylanase; Marine enzymes |
| Auteurs | | Top |
- Martin, M., meer
- Vandermies, M.
- Joyeux, C., meer
- Martin, R., meer
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- Barbeyron, T.
- Michel, G.
- Vandenbol, M., meer
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| Abstract |
Alga-associated microorganisms, in the context of their numerous interactions with the host and the complexity of the marine environment, are known to produce diverse hydrolytic enzymes with original biochemistry. We recently isolated several macroalgal-polysaccharide-degrading bacteria from the surface of the brown alga Ascophyllum nodosum. These active isolates belong to two classes: the Flavobacteriia and the Gammaproteobacteria. In the present study, we constructed two “plurigenomic” (with multiple bacterial genomes) libraries with the 5 most interesting isolates (regarding their phylogeny and their enzymatic activities) of each class (Fv and Gm libraries). Both libraries were screened for diverse hydrolytic activities. Five activities, out of the 48 previously identified in the natural polysaccharolytic isolates, were recovered by functional screening: a xylanase (GmXyl7), a beta-glucosidase (GmBg1), an esterase (GmEst7) and two iota-carrageenases (Fvi2.5 and Gmi1.3). We discuss here the potential role of the used host-cell, the average DNA insert-sizes and the used restriction enzymes on the divergent screening yields obtained for both libraries and get deeper inside the “great screen anomaly”. Interestingly, the discovered esterase probably stands for a novel family of homoserine o-acetyltransferase-like-esterases, while the two iota-carrageenases represent new members of the poorly known GH82 family (containing only 19 proteins since its description in 2000). These original results demonstrate the efficiency of our uncommon “plurigenomic” library approach and the underexplored potential of alga-associated cultivable microbiota for the identification of novel and algal-specific enzymes. |
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