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Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas
Clark, M.S.; Thorne, M.A.S.; Amaral, A.; Vieira, F.; Batista, F.M.; Reis, J.; Power, D.M. (2013). Identification of molecular and physiological responses to chronic environmental challenge in an invasive species: the Pacific oyster, Crassostrea gigas. Ecol. Evol. 3(10): 3283-3297. https://dx.doi.org/10.1002/ece3.719
In: Ecology and Evolution. John Wiley & Sons: Chichester. ISSN 2045-7758; e-ISSN 2045-7758, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Climate Change
    Environmental Managers & Monitoring
    Marine Sciences > Biodiversity
    Marine Sciences > Marine Genomics
    Scientific Community
    Scientific Publication
    Marien/Kust
Author keywords
    Candidate genes; condition index; energetic trade-offs; mTOR pathway;transcriptional profiling
Project Top | Auteurs 
  • Association of European marine biological laboratories, meer
Auteurs  Top 
  • Clark, M.S.
  • Thorne, M.A.S.
  • Amaral, A.
  • Vieira, F.
  • Batista, F.M.
  • Reis, J.
  • Power, D.M.
Abstract
    Understanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, Crassostrea gigas, a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3-month laboratory-based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH. Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI3K/AKT/mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C. gigas which could significantly impact on coastal ecosystem biodiversity and function in the future.
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