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Anaerobic oxidation of ethane by archaea from a marine hydrocarbon seep
Chen, S.-C.; Musat, N.; Lechtenfeld, O.J.; Paschke, H.; Schmidt, M.; Said, N.; Popp, D.; Calabrese, F.; Stryhanyuk, H.; Jaekel, U.; Zhu, Y.-G.; Joye, S.B.; Richnow, H.-H.; Widdel, F.; Musat, F. (2019). Anaerobic oxidation of ethane by archaea from a marine hydrocarbon seep. Nature (Lond.) 568(7750): 108-111. https://dx.doi.org/10.1038/s41586-019-1063-0
In: Nature: International Weekly Journal of Science. Nature Publishing Group: London. ISSN 0028-0836; e-ISSN 1476-4687, meer
Is een onderreeks van:
Ragsdale, S.W. (2019). A microbe that eats ethane under the sea. Nature (Lond.) 568(7750): 40-41, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Auteurs  Top 
  • Chen, S.-C.
  • Musat, N.
  • Lechtenfeld, O.J.
  • Paschke, H.
  • Schmidt, M.
  • Said, N.
  • Popp, D.
  • Calabrese, F.
  • Stryhanyuk, H.
  • Jaekel, U.
  • Zhu, Y.-G.
  • Joye, S.B.
  • Richnow, H.-H.
  • Widdel, F., meer
  • Musat, F.
Abstract
    Ethane is the second most abundant component of natural gas in addition to methane, and—similar to methane—is chemically unreactive. The biological consumption of ethane under anoxic conditions was suggested by geochemical profiles at marine hydrocarbon seeps, and through ethane-dependent sulfate reduction in slurries. Nevertheless, the microorganisms and reactions that catalyse this process have to date remained unknown. Here we describe ethane-oxidizing archaea that were obtained by specific enrichment over ten years, and analyse these archaea using phylogeny-based fluorescence analyses, proteogenomics and metabolite studies. The co-culture, which oxidized ethane completely while reducing sulfate to sulfide, was dominated by an archaeon that we name ‘Candidatus Argoarchaeum ethanivorans’; other members were sulfate-reducing Deltaproteobacteria. The genome of Ca. Argoarchaeum contains all of the genes that are necessary for a functional methyl-coenzyme M reductase, and all subunits were detected in protein extracts. Accordingly, ethyl-coenzyme M (ethyl-CoM) was identified as an intermediate by liquid chromatography–tandem mass spectrometry. This indicated that Ca. Argoarchaeum initiates ethane oxidation by ethyl-CoM formation, analogous to the recently described butane activation by ‘Candidatus Syntrophoarchaeum’. Proteogenomics further suggests that oxidation of intermediary acetyl-CoA to CO2 occurs through the oxidative Wood–Ljungdahl pathway. The identification of an archaeon that uses ethane (C2H6nH2n+2) without oxygen. Detection of phylogenetic and functional gene markers related to those of Ca. Argoarchaeum at deep-sea gas seeps suggests that archaea that are able to oxidize ethane through ethyl-CoM are widespread members of the local communities fostered by venting gaseous alkanes around these seeps.
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