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The use of high pressure liquid chromatography with fluorimetric detection for chlorophyll A determination in natural extracts of chloropigments and their degradation products
Goeyens, L.; Post, E.; Dehairst, F.; Vandenhoudt, A.; Baeyens, W.F.J. (1982). The use of high pressure liquid chromatography with fluorimetric detection for chlorophyll A determination in natural extracts of chloropigments and their degradation products. Int. J. Environ. Anal. Chem. 12: 51-63
In: International journal of environmental and analytical chemistry. Taylor & Francis: Amsterdam. ISSN 0306-7319; e-ISSN 1029-0397, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Chromatography
    Organic compounds > Carbohydrates > Glycosides > Pigments > Photosynthetic pigments > Chlorophylls
    Marien/Kust
Auteurs  Top 
  • Goeyens, L., meer
  • Post, E.
  • Dehairst, F.
  • Vandenhoudt, A.
  • Baeyens, W.F.J., meer
Abstract
    To realize chlorophyll A analysis, a method is proposed that combines the sensitivity of fluorimetric determinations and the inherent selectivity and accuracy of chromatographic separations. A partial application of the overlapping resolution mapping (ORM) method led to the use of a methanol:acetone:water (75:22:3, v/v) mixture for the isocratic separation by high pressure liquid chromatography on a reserved phase C-18 column of at least sixteen chloropigments and degradation products in a natural extract. Ten replicate measurements of a 50 µg.l-1 standard showed a reproducibility of 3 promille while a detection limit of 84 pg chlorophyll A was estimated.
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