Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
Biosynthesis of ditropolonyl sulfide, an antibacterial compound produced by Burkholderia cepacia complex strain R-12632
Depoorter, E.; Coenye, T.; Vandamme, P. (2021). Biosynthesis of ditropolonyl sulfide, an antibacterial compound produced by Burkholderia cepacia complex strain R-12632. Appl. Environ. Microbiol. 87(22): e01169-21. https://dx.doi.org/10.1128/aem.01169-21
In: Applied and Environmental Microbiology. American Society for Microbiology: Washington. ISSN 0099-2240; e-ISSN 1098-5336, meer
|  |
| Trefwoorden |
Prokaryotes > Microorganisms > Bacteria > Gracilicutes > Burkholderiaceae > Burkholderia > Plant pathogenic bacteria > Burkholderia cepacia
Marien/Kust |
| Author keywords |
Burkholderia cepacia complex, ditropolonyl sulfide, natural products, transposon mutagenesis, antibacterial activity |
| Abstract |
Burkholderia cepacia complex strain R-12632 produces ditropolonyl sulfide, an unusual sulfur-containing tropone, via a yet-unknown biosynthetic pathway. Ditropolonyl sulfide purified from a culture of strain R-12632 inhibits the growth of various Gram-positive and Gram-negative resistant bacteria, with MIC values as low as 16 μg/ml. In the present study, we used a transposon mutagenesis approach combined with metabolite analyses to identify the genetic basis for antibacterial activity of strain R-12632 against Gram-negative bacterial pathogens. Fifteen of the 8304 transposon mutants investigated completely lost antibacterial activity against Klebsiella pneumoniae LMG 2095. In these loss-of-activity mutants, nine genes were interrupted. Four of those genes were involved in assimilatory sulfate reduction, two were involved in phenylacetic acid (PAA) catabolism, and one was involved in glutathione metabolism. Via semipreparative fractionation and metabolite identification, it was confirmed that inactivation of the PAA degradation pathway or glutathione metabolism led to loss of ditropolonyl sulfide production. Based on earlier studies on the biosynthesis of tropolone compounds, the requirement for a functional PAA catabolic pathway for antibacterial activity in strain R-12632 indicated that this pathway likely provides the tropolone backbone for ditropolonyl sulfide. Loss of activity observed in mutants defective in assimilatory sulfate reduction and glutathione biosynthesis suggested that cysteine and glutathione are potential sources of the sulfur atom linking the two tropolone moieties. The demonstrated antibacterial activity of the unusual antibacterial compound ditropolonyl sulfide warrants further studies into its biosynthesis and biological role. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
