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Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin Paracentrotus lividus
Pjeta, R.; Lindner, H.; Kremser, L.; Salvenmoser, W.; Sobral, D.; Ladurner, P.; Santos, R. (2020). Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin Paracentrotus lividus. International Journal of Molecular Sciences 21(3): 946. https://dx.doi.org/10.3390/ijms21030946
In: International Journal of Molecular Sciences. MDPI AG: Basel. ISSN 1661-6596; e-ISSN 1422-0067, meer
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| Trefwoorden |
Echinodermata [WoRMS]; Paracentrotus lividus (Lamarck, 1816) [WoRMS]
Marien/Kust |
| Author keywords |
echinoderm; sea urchin; bioadhesion; differential RNAseq; mass spectrometry; in situ hybridization |
| Auteurs | | Top |
- Pjeta, R.
- Lindner, H.
- Kremser, L.
- Salvenmoser, W.
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- Sobral, D.
- Ladurner, P.
- Santos, R., meer
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| Abstract |
Echinoderms, such as the rock-boring sea urchin Paracentrotus lividus, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of the adhesive, as well as the releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset from Lebesgue et al. by mapping mass spectrometry-derived peptides to a P. lividus de novo transcriptome generated in this study. This resulted in a drastic increase in mapped proteins in comparison to the previous publication. The data were subsequently combined with a differential RNAseq approach to identify potential adhesion candidate genes. A gene expression analysis of 59 transcripts using whole mount in situ hybridization led to the identification of 16 transcripts potentially involved in bioadhesion. In the future these data could be useful for the production of synthetic reversible adhesives for industrial and medical purposes. |
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