Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
A modified staining protocol to study early development of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) skull
Goharimanesh, M.; Yazdani Moghaddam , F.; Mahdavi Shahri, N.; Ghassemzadeh, F. (2020). A modified staining protocol to study early development of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) skull. Iran. J. Fish. Sci. 19(5): 2501-2511. https://dx.doi.org/10.22092/ijfs.2019.119342
In: Iranian Journal of Fisheries Sciences. Iranian Fisheries Research Organization: Tehran. ISSN 1562-2916; e-ISSN 2322-5696, meer
|   |
| Author keywords |
Alcian blue, Alizarin red, Cartilaginous and bony tissues, Skeletal development, Staining |
| Auteurs | | Top |
- Goharimanesh, M., meer
- Yazdani Moghaddam , F.
- Mahdavi Shahri, N.
- Ghassemzadeh, F.
|
|
|
| Abstract |
Alcian blue binds to the proteoglycans components of the extracellular matrix in chondrocytes and turns the cartilage color to blue. Alizarin red stains free calcium and certain calcium compound to a red or light purple color. This procedure is a useful tool to study the skeletal development in several fish species and then to precociously diagnose malformations in cartilages and bones development in small larvae and eventually to better characterize the effect of different environmental and/or nutritional factors on the ossification status of specific skeletal components. It should be noted that according to different species, the modifications in the protocol are still reported. In this experiment 520 specimens of rainbow trout in different life stages from one-day post hatching (dph) to 30 days, 1.5±0.1 and 3.5±0.5 cm in length have been studied. The double staining protocol for developing rainbow trout has been defined based on diverse published protocols. For an optimal staining protocol design, the incubation times of the different solutions were adjusted. Then the photos of stained specimens have been used to study and compare. The detection of cartilages and bones helps to understand the ontogeny of fish skeletal structure and the effects of environmental factors on bone density and plasticity. In addition, given the importance of skeletal and muscle's interactions, it is possible to conduct a survey on specific factors to change the skeletal tissues. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
