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Compound-specific isotope analysis of amino acids for aquatic systems – Problems, challenges, solutions: A review
Khaliq, S.; Jochmann, M.A.; Hesse, T.; Nachev, M.; Sures, B.; Riekenberg, P.M.; van der Meer, M.T.J.; Schmidt, T.C. (2024). Compound-specific isotope analysis of amino acids for aquatic systems – Problems, challenges, solutions: A review. Trends Anal. Chem. 181: 118038. https://dx.doi.org/10.1016/j.trac.2024.118038
In: Trends in Analytical Chemistry. Elsevier: Amsterdam. ISSN 0165-9936; e-ISSN 1879-3142, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Author keywords
    Compound-specific stable isotope analysis; CSIA; Amino acids; Aquatic systems; GC-IRMS; LC-IRMS
Auteurs  Top 
  • Khaliq, S.
  • Jochmann, M.A.
  • Hesse, T.
  • Nachev, M.
  • Sures, B.
  • Riekenberg, P.M., meer
  • van der Meer, M.T.J.
  • Schmidt, T.C.
Abstract
    Compound-specific isotope analysis of individual amino acids (AAs) is a promising method for characterizing resource flow and transformation in aquatic systems, encompassing microbial domains to macroscopic consumers. This review delves into two key areas of research concerning AAs in aquatic ecosystems. Firstly, carbon isotope analysis is employed to explore carbon sources assimilated for AAs synthesis, and secondly, nitrogen isotope analysis is used to clarify ecosystem baselines and trophic dynamics of organisms. Surprisingly, literature provides limited discussions on the limitations or critical challenges faced during AAs isotope analysis over the past decade. To address this gap, this review focuses on challenges encountered in method development, sample preparation, and technical aspects, including instrumental design, and δ13C and δ15N analysis of AAs. Recommendations are provided to increase the accuracy and reliability. The review also highlights the significance of comprehensive understanding and detailed methodology to address challenges during AA isotope analysis in aquatic systems.
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