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y-glutamyl transpeptidase as a possible marker of sertoli cells in testes of fish
Kinnberg, K.; Christiansen, T.; Bjerregaard, P.; Korsgaard, B. (2000). y-glutamyl transpeptidase as a possible marker of sertoli cells in testes of fish, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 387
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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| Auteurs | | Top |
- Kinnberg, K.
- Christiansen, T., meer
- Bjerregaard, P.
- Korsgaard, B.
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| Abstract |
Finding markers directly correlating effects of estrogenic chemicals with the reproductive system of male fish is of great importance. In fish, gamma -glutamyl transpeptidase ( gamma -GTP) has been localised in various tissues, but not in testes. However, in testes of mammals gamma -GTP has been localised to Sertoli cells and used as a Sertoli cell marker. If this also applies to fish, gamma -GTP could be used as a marker of effects on Sertoli cells for studies of estrogenic chemicals. In our earlier studies nonylphenol treatment of eelpout resulted in a marked reduction in testicular gamma -GTP activity concomitant with a reduction in Sertoli cell size. In contrast, treatment of platyfish resulted in a tendency of increased gamma -GTP activity concomitant with a hypertrophy of Sertoli cells. To study our hypothesis of testicular gamma -GTP being a Sertoli cell marker we have examined the relationship between the histological structure of Sertoli cells and gamma -GTP activity in 6 fish species. |
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