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Selected bacterial strains protect Artemia spp. from the pathogenic effects of Vibrio proteolyticus CW8T2
Verschuere, L.; Heang, H.; Criel, G.R.J.; Sorgeloos, P.; Verstraete, W. (2000). Selected bacterial strains protect Artemia spp. from the pathogenic effects of Vibrio proteolyticus CW8T2. Appl. Environ. Microbiol. 66(3): 1139-1146. https://dx.doi.org/10.1128/AEM.66.3.1139-1146.2000
In: Applied and Environmental Microbiology. American Society for Microbiology: Washington. ISSN 0099-2240; e-ISSN 1098-5336, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Analysis > Microbiological analysis
    Control > Biological control
    Control > Disease control
    Cultures > Shellfish culture > Crustacean culture > Brine shrimp culture
    Diseases > Infectious diseases
    Fauna > Aquatic organisms > Aquatic animals
    Microorganisms > Bacteria
    Pathogens > Bacteria > Pathogenic bacteria
    Artemia Leach, 1819 [WoRMS]; Vibrio proteolyticus (Merkel et al., 1964) Baumann et al., 1982 [WoRMS]
    Marien/Kust; Brak water
Auteurs  Top 
  • Verschuere, L.
  • Heang, H.
  • Criel, G.R.J., meer
  • Sorgeloos, P., meer
  • Verstraete, W., redacteur, meer
Abstract
    In this study Vibrio proteolyticus CW8T2 has been identified as a virulent pathogen for Artemia spp. Its infection route has been visualized with transmission electron microscopy. The pathogen affected microvilli and gut epithelial cells, disrupted epithelial cell junctions, and reached the body cavity, where it devastated cells and tissues. In vivo antagonism tests showed that pre-emptive colonization of the culture water with nine selected bacterial strains protected Artemia juveniles against the pathogenic effects. Two categories of the selected strains could be distinguished: (i) strains providing total protection, as no mortality occurred 2 days after the experimental infection with V. proteolyticus CW8T2, with strain LVS8 as a representative, and (ii) strains providing partial protection, as significant but not total mortality was observed, with strain LVS2 as a representative. The growth of V. proteolyticus CW8T2 in the culture medium was slowed down in the presence of strains LVS2 and LVS8, but growth suppression was distinctly higher with LVS8 than with LVS2. It was striking that the strains that gave only partial protection against the pathogen in the in vivo antagonism test showed also a restricted capability to colonize the Artemia compared to the strains providing total protection. The in vivo antagonism tests and the filtrate experiments showed that probably no extracellular bacterial compounds were involved in the protective action but that the living cells were required to protect Artemia against V. proteolyticus CW8T2.
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