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Evaluation of different yeast cell wall mutants and microalgae strains as feed for gnotobiotically grown brine shrimp Artemia franciscana
Marques, A.; Dhont, J.; Sorgeloos, P.; Bossier, P. (2004). Evaluation of different yeast cell wall mutants and microalgae strains as feed for gnotobiotically grown brine shrimp Artemia franciscana. J. Exp. Mar. Biol. Ecol. 312(1): 115-136. dx.doi.org/10.1016/j.jembe.2004.06.008
In: Journal of Experimental Marine Biology and Ecology. Elsevier: New York. ISSN 0022-0981; e-ISSN 1879-1697, meer
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| Trefwoorden |
Artemia Leach, 1819 [WoRMS]; Artemia franciscana Kellog, 1906 [WoRMS]; Dunaliella tertiolecta Butcher, 1959 [WoRMS]; Saccharomyces cerevisiae Meyen ex E.C. Hansen, 1883 [WoRMS]
Marien/Kust |
| Author keywords |
Artemia; gnotobiotic culture; Saccharomyces cerevisiae; Dunaliella tertiolecia |
| Abstract |
The nutritional value of isogenic yeast strains and two microalgal species for gnotobiotically grown Artemia was examined. Yeast cell wall mutants were always better feed for Artemia than their respective wild type. Yeast cells harbouring null mutants for enzymes involved early in the biochemical pathway for cell wall mannoproteins synthesis performed best as feed for Artemia. Yeast cells defective in chitin or ß-glucan production were scored in second order. The mnn6 isogenic yeast mutant, harbouring a null mutation for mannoprotein phosphorylation, performed poorly as feed for Artemia, although with good growth. These results suggest that any mutation affecting the yeast cell wall scaffolding by reducing the amount of covalent links between the major components of yeast cell wall, namely mannoproteins, ß-glucans and chitin, is sufficient to improve the digestibility for Artemia. The results with microalgae indicated that within one species, strains can have different nutritional value under gnotobiotic conditions. The growth phase was another parameter influencing feed quality, although here it was not possible to reveal the exact cause. It is anticipated that the standard Artemia gnotobiotic growth test is an excellent tool to study the mode of action of bacteria, with a probiotic as well as with a pathogenic character. |
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