Open WL Archief (OWA)

Over het archief

Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.

Basisinformatie over ‘Open Access to scholarly information'.

[ meld een fout in dit record ]

mandje (1): toevoegen | toon

---

one publication added to basket [111935]

Species-specific probe, based on 18S rDNA sequence, could be used for identification of the mucilage producer microalga Gonyaulax fragilis (Dinophyta) Tinti, F.; Boni, L.; Pistocchi, R.; Riccardi, M.; Guerrini, F. (2007). Species-specific probe, based on 18S rDNA sequence, could be used for identification of the mucilage producer microalga Gonyaulax fragilis (Dinophyta), in: Relini, G. et al. Biodiversity in enclosed seas and artificial marine habitats: Proceedings of the 39th European Marine Biology Symposium, held in Genoa, Italy, 21-24 July 2004. Developments in Hydrobiology, 193: pp. 259-263. https://dx.doi.org/10.1007/978-1-4020-6156-1_24 In: Relini, G.; Ryland, J. (Ed.) (2007). Biodiversity in enclosed seas and artificial marine habitats: Proceedings of the 39th European Marine Biology Symposium, held in Genoa, Italy, 21-24 July 2004. European Marine Biology Symposia, 39. Developments in Hydrobiology, 193. ISBN 978-1-4020-6155-4; e-ISBN 978-1-4020-6156-1. VII, 271 pp. https://dx.doi.org/10.1007/978-1-4020-6156-1, meer In: European Marine Biology Symposia., meer Is gerelateerd aan: Tinti, F.; Boni, L.; Pistocchi, R.; Riccardi, M.; Guerrini, F. (2007). Species-specific probe, based on 18S rDNA sequence, could be used for identification of the mucilage producer microalga Gonyaulax fragilis (Dinophyta). Hydrobiologia 580(1): 259-263. https://dx.doi.org/10.1007/s10750-006-0446-z, meer

---

Beschikbaar in	Auteurs
VLIZ [ aanvragen ]
Documenttype: Congresbijdrage

---

Trefwoorden

Chemical compounds > Organic compounds > Proteins > Mucins Gonyaulax fragilis (Schütt) Kofoid, 1911 [WoRMS] MED, Adriatic [Marine Regions] Marien/Kust

Author keywords

Gonyaulax fragilis; 18S rDNA; mucilage; Adriatic Sea

---

Auteurs		Top
Tinti, F., meer Boni, L., meer Pistocchi, R.	Riccardi, M. Guerrini, F.

---

Abstract

In the Adriatic Sea, the correlation between mucilage phenomena and the presence of Gonyaulax fragilis (Schütt) Kofoid (Dinophyta) has been recently demonstrated. The application of PCR-based methods and the development of species-specific molecular probes might represent powerful technologies for rapid and specific monitoring of microalgal species in seawater samples. Here, we report sequencing of the small subunit (SSU) ribosomal RNA gene (18S rDNA) of G. fragilis and its comparative analysis within the Dinophyta. Total DNAs were extracted and amplified from cultured cells of G. fragilis, which were isolated from natural phytoplanktonic association in the northern Adriatic Sea. Total 18S rDNA gene was amplified using 16S1N and 16S2N primers and sequenced using ad hoc designed internal primers. The primers amplified a product of expected size (length 1700/1800 bp). The phylogenetic analysis carried out by comparing G. fragilis sequence to homologous sequences of Lingulodinium polyedrum (Stein) Dodge, Gonyaulax spinifera (Claparède et Lachmann) Diesing, Protoceratium reticulatum (Claparède et Lachmann) Bütschli revealed a great nucleotide divergence of G. fragilis SSU sequence. Therefore, the SSU sequence could be used as species-specific marker for the identification of this mucilage producer microalga. In addition, such sequence could be used as target to design oligonucleotide probes for the construction of DNA microchips as diagnostic tool for the routine monitoring of harmful algae in seawater.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs

IMIS is ontwikkeld en wordt gehost door het VLIZ.