Open WL Archief (OWA)

Over het archief

Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.

Basisinformatie over ‘Open Access to scholarly information'.

[ meld een fout in dit record ]

mandje (1): toevoegen | toon

---

one publication added to basket [17400]

Quantitative analysis of turbellarian cell suspension by fluorescent staining with acridine orange, and video microscopy Behensky, C.; Schürmann, W.; Peter, R. (2001). Quantitative analysis of turbellarian cell suspension by fluorescent staining with acridine orange, and video microscopy. Belg. J. Zool. 131(Suppl. 1): 131-136 In: Belgian Journal of Zoology. Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Gent. ISSN 0777-6276; e-ISSN 2295-0451, meer Ook verschenen in: Saló, E.; Watson, N.; Schockaert, E. (Ed.) (2001). Proceedings of the 9th International Symposium on the Biology of the Turbellaria, Barcelona, Spain, June 2000. Belgian Journal of Zoology, 131(Suppl. 1). Koninklijke Belgische Vereniging voor Dierkunde = Société royale zoologique de Belgique: Diepenbeek. 236 pp., meer

---

Beschikbaar in	Auteurs
VLIZ: Open access 358137 [ download pdf ]

---

Trefwoorden

Acids > Organic compounds > Organic acids > Nucleic acids > DNA Acids > Organic compounds > Organic acids > Nucleic acids > RNA Analysis > Image analysis Analytical techniques > Microscopy > Fluorescence microscopy Biological phenomena > Regeneration Cell constituents > Chromosomes > Genes Cultures > Cell suspensions Image analysis Image interpretation > Image analysis Morphogenesis Toxicology > Toxic substances > Mutagens > Acridines > Acridine orange Macrostomum Schmidt, 1848 [WoRMS]; Platyhelminthes [WoRMS] Marien/Kust

---

Auteurs		Top
Behensky, C. Schürmann, W. Peter, R.

---

Abstract

A combination of methods has been developed for analyzing cell suspensions from turbellarians with respect to cytochemical and morphometric parameters and with special emphasis on the characterization of neoblasts. Tissues were disintegrated by mechanical and enzymatic means. Neoblasts were separated and/or fractionated by different centrifugation protocols in Percoll density-gradients. Staining with acridine orange under conditions denaturing RNA but leaving intact double stranded DNA yielded green fluorescence of DNA and red phosphorescence of RNA. Both light emissions were documented and quantitative image analyses were performed. By computing the integral intensities of green and red light, quantitative measures were gained for DNA and RNA contents. By correlating these light emissions to each other or to cell size, histograms characteristic for given neoblast pools were obtained. The protocol described should facilitate monitoring the heterogeneity of the neoblast compartment as well as studying cellular dynamics during growth, regeneration and other physiological processes.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs

IMIS is ontwikkeld en wordt gehost door het VLIZ.