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Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays
Vanderperren, E.; Demaré, W.; Blust, R.; Cooreman, K.; Bossier, P. (2001). Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays. Biomed. Lett. 6(5): 375-380. https://dx.doi.org/10.1080/13547500110044258
In: Biomedical letters. Faculty Press: Cambridge. ISSN 0961-088X, meer
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| Abstract |
The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use of recombinant yeast cells that harbour an oestrogen receptor expression cassette and a reporter construct, coding for beta -galactosidase. The induction mechanism starts with the binding of oestrogenic compounds to the oestrogen receptor. This complex activates the production of beta -galactosidase. The beta -galactosidase activity is thus a measure of the oestrogenic activity of chemical compounds. In the YES assay, the beta -galactosidase activity may be quantified with the chromogenic substrate chlorophenol red-beta -D-galactopyranoside (CPRG). In the present study it is reported that CPRG or its beta -galactosidase degradation product chlorophenol red act in the YES as an oestrogenic compound itself. The implications of this finding are described. It is especially argued that chlorophenol red production after prolonged incubation of the assay might be misinterpreted as an oestrogenic effect of the test compound. |
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