Open WL Archief (OWA)

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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.

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one publication added to basket [20167]
Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka Abeysinghe, P.D.; Triest, L.; De Greef, B.; Koedam, N.; Hettiarachi, S. (1999). Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka. Hydrobiologia 413: 11-16. https://dx.doi.org/10.1023/A:1003899028558 In: Hydrobiologia. Springer: The Hague. ISSN 0018-8158; e-ISSN 1573-5117, meer

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Beschikbaar in	Auteurs
VLIZ: Open Marine Archive 280462 [ download pdf ]

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Trefwoorden

Acids > Organic compounds > Organic acids > Nucleic acids > DNA Biology > Genetics > Population genetics Distribution > Geographical distribution Genetic parameters > Genetic variance Markers > Genetic markers > Dna > Random amplified polymorphic dna Natural populations > Plant populations Polymerase chain reaction Subpopulations Water bodies > Inland waters > Wetlands > Swamps > Mangrove swamps Bruguiera gymnorhiza (L.) Lamk. [WoRMS]; Bruguiera sexangula (Lour.) Poir. [WoRMS] Marien/Kust; Brak water

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Auteurs		Top
Abeysinghe, P.D., meer Triest, L., meer De Greef, B.	Koedam, N., meer Hettiarachi, S.

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Abstract

The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened, 20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a small number of primers.

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