Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [205485] |
Molecular diversity in a hydrogen-consuming enrichment culture from the South Pacific gyre
Mardhiah, U. (2010). Molecular diversity in a hydrogen-consuming enrichment culture from the South Pacific gyre. MSc Thesis. Max Planck Institute for Marine Microbiology/Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC): Bremen. 47 pp.
|
| Beschikbaar in | Auteur |
|
Documenttype: Doctoraat/Thesis/Eindwerk
|
| Abstract |
The South Pasific Gyre is known to be the most oligotrophic water body on earth. Due to a low a very low amount of organic carbon sediments and thus oxygen penetrates deeply into the sediment. Hydrogen produced through natural radiation and radiolysis of water was hypothesized to be a major source of energy for sediment microorganisms. During the Knox-02RR cruise in 2007, the first centimetres of this sediment were sampled and oxic slurries were enriched with an over pressure of H2/CO2 (80:20) for 22 months at 4°C. Gas was consumed after this incubation suggesting the activity of Knallgas microorganisms. The microbial community was investigated with a 16S rRNA gene libraries and by T-RFLP. Analysis of 16S rRNA gene showed that only the genus Halomonas was found in the enrichment. This was confirmed by T-RFLP using the restriction enzyme EcoRI, HhaI and SacII. Isolation attempts yielded strains that affiliated to Halomonas. Degenerated primers for a hydrogen subunit did not enable the amplification of a hydrogenase gene. These results suggest a utilisation by Halomonas, eventually by a novel hydrogenase system |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
