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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
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| one publication added to basket [211053] |
Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes
Janssens, B.J.; Le Gall, R.; Rees, J.F. (2002). Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes. J. Fish Biol. 61(1): 71-84. dx.doi.org/10.1006/jfbi.2002.2008
In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112; e-ISSN 1095-8649, meer
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| Trefwoorden |
Dicentrarchus labrax (Linnaeus, 1758) [WoRMS]
Marien/Kust |
| Author keywords |
sea bass; erythrocytes; oxidative stress; peroxide; glutathione; DNA |
| Auteurs | | Top |
- Janssens, B.J.
- Le Gall, R.
- Rees, J.F., meer
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| Abstract |
The haemolysis of sea bass Dicentrarchus labrax red blood cells (RBC) was initiated by tert-butyl-hydroperoxide (t-BHP). The onset of the haemolytic process, as accelerated by increasing t-BHP concentration, This process as preceded by a drop in the RBC glutathione content followed by the production of lipid peroxidation products. Also t-BHP induced DNA fragmentation in RBC nuclei as measured by COMET assay. The addition of the antioxidant Trolox C-R dose-dependent delayed the onset of both lipid peroxidation and haemolysis, and protected GSH stores against t-BHP-induced depletion. DNA fragmentation was also prevented by Trolox C-R. These results indicate that L-BHP induces haemolysis in sea bass RBC through the induction of oxidative stress Such a simple model Could prove useful for both fundamental and applied studies on marine fish antioxidant mechanisms. |
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