Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [218509] |
Oligopeptide repeats in the yeast protein Sup35p stabilize intermolecular prion interactions
Parham, S.N.; Resende, C.G.; Tuite, M.F. (2001). Oligopeptide repeats in the yeast protein Sup35p stabilize intermolecular prion interactions. EMBO J. 20(9): 2111-2119. http://dx.doi.org/10.1093/emboj/20.9.2111
In: The EMBO Journal. Nature Publishing Group: Eynsham (P.O. Box 1, Eynsham, Oxford OX8 1JJ). ISSN 0261-4189; e-ISSN 1460-2075, meer
|   |
| Trefwoord |
|
| Author keywords |
oligopeptide repeats - prion - PrP - Sup35p - yeast |
| Auteurs | | Top |
- Parham, S.N.
- Resende, C.G., meer
- Tuite, M.F.
|
|
|
| Abstract |
The nuclear-encoded Sup35p protein is responsible for the prion-like [PSI+] determinant of yeast, with Sup35p existing largely as a high molecular weight aggregate in [PSI+] strains. Here we show that the five oligopeptide repeats present at the N-terminus of Sup35p are responsible for stabilizing aggregation of Sup35p in vivo. Sequential deletion of the oligopeptide repeats prevented the maintenance of [PSI+] by the truncated Sup35p, although deletants containing only two repeats could be incorporated into pre-existing aggregates of wild-type Sup35p. The mammalian prion protein PrP also contains similar oligopeptide repeats and we show here that a human PrP repeat (PHGGGWGQ) is able functionally to replace a Sup35p oligopeptide repeat to allow stable [PSI+] propagation in vivo. Our data suggest a model in which the oligopeptide repeats in Sup35p stabilize intermolecular interactions between Sup35p proteins that initiate establishment of the aggregated state. Modulating repeat number therefore alters the rate of yeast prion conversion in vivo. Furthermore, there appears to be evolutionary conservation of function of the N-terminally located oligopeptide repeats in prion propagation. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
