Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [238166] |
BcsTx3 is a founder of a novel sea anemone toxin family of potassium channel blocker
Orts, D.J.B.; Moran, Y.; Cologna, C.T.; Peigneur, S.; Madio, B.; Praher, D.; Quinton, L.; De Pauw, E.; Bicudo, J.E.P.W.; Tytgat, J.; de Freitas, J.C. (2013). BcsTx3 is a founder of a novel sea anemone toxin family of potassium channel blocker. The FEBS Journal 280(19): 4839-4852. dx.doi.org/10.1111/febs.12456
In: The FEBS Journal. Wiley-Blackwell: Oxford. ISSN 1742-464X; e-ISSN 1742-4658, meer
|  |
| Trefwoorden |
Bunodosoma caissarum Corrêa in Belém, 1987 [WoRMS]
Marien/Kust |
| Author keywords |
Bunodosomacaissarum; potassium channel toxin; Saint Peter and Saint PaulArchipelago; sea anemone; sequence alignment |
| Auteurs | | Top |
- Orts, D.J.B.
- Moran, Y.
- Cologna, C.T., meer
- Peigneur, S., meer
|
- Madio, B.
- Praher, D.
- Quinton, L., meer
- De Pauw, E., meer
|
- Bicudo, J.E.P.W.
- Tytgat, J., meer
- de Freitas, J.C.
|
| Abstract |
Sea anemone venoms have become a rich source of peptide toxins which are invaluable tools for studying the structure and functions of ion channels. In this work, BcsTx3, a toxin found in the venom of a Bunodosoma caissarum (population captured at the Saint Peter and Saint Paul Archipelago, Brazil) was purified and biochemically and pharmacologically characterized. The pharmacological effects were studied on 12 different subtypes of voltage-gated potassium channels (KV1.1–KV1.6; KV2.1; KV3.1; KV4.2; KV4.3; hERG and Shaker IR) and three cloned voltage-gated sodium channel isoforms (NaV1.2, NaV1.4 and BgNaV1.1) expressed in Xenopus laevis oocytes. BcsTx3 shows a high affinity for Drosophila Shaker IR channels over rKv1.2, hKv1.3 and rKv1.6, and is not active on NaV channels. Biochemical characterization reveals that BcsTx3 is a 50 amino acid peptide crosslinked by four disulfide bridges, and sequence comparison allowed BcsTx3 to be classified as a novel type of sea anemone toxin acting on KV channels. Moreover, putative toxins homologous to BcsTx3 from two additional actiniarian species suggest an ancient origin of this newly discovered toxin family. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
