Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [250538] |
Detection of anthropogenic particles in fish stomachs: An isolation method adapted to identification by Raman spectroscopy
Collard, F.; Gilbert, B.; Eppe, G.; Parmentier, E.; Das, K. (2015). Detection of anthropogenic particles in fish stomachs: An isolation method adapted to identification by Raman spectroscopy. Arch. Environ. Contam. Toxicol. 69(3): 331-339. https://dx.doi.org/10.1007/s00244-015-0221-0
In: Archives of Environmental Contamination and Toxicology. Springer: New York. ISSN 0090-4341; e-ISSN 1432-0703, meer
|  |
| Auteurs | | Top |
- Collard, F., meer
- Gilbert, B.
- Eppe, G., meer
|
|
|
| Abstract |
Microplastic particles (MP) contaminate oceans and affect marine organisms in several ways. Ingestion combined with food intake is generally reported. However, data interpretation often is circumvented by the difficulty to separate MP from bulk samples. Visual examination often is used as one or the only step to sort these particles. However, color, size, and shape are insufficient and often unreliable criteria. We present an extraction method based on hypochlorite digestion and isolation of MP from the membrane by sonication. The protocol is especially well adapted to a subsequent analysis by Raman spectroscopy. The method avoids fluorescence problems, allowing better identification of anthropogenic particles (AP) from stomach contents of fish by Raman spectroscopy. It was developed with commercial samples of microplastics and cotton along with stomach contents from three different Clupeiformes fishes: Clupea harengus, Sardina pilchardus, and Engraulis encrasicolus. The optimized digestion and isolation protocol showed no visible impact on microplastics and cotton particles while the Raman spectroscopic spectrum allowed the precise identification of microplastics and textile fibers. Thirty-five particles were isolated from nine fish stomach contents. Raman analysis has confirmed 11 microplastics and 13 fibers mainly made of cellulose or lignin. Some particles were not completely identified but contained artificial dyes. The novel approach developed in this manuscript should help to assess the presence, quantity, and composition of AP in planktivorous fish stomachs. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
