Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [257059] |
Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage
Huysman, M.J.J.; Tanaka, A.; Bowler, C.; Vyverman, W.; De Veylder, L. (2015). Functional characterization of the diatom cyclin-dependent kinase A2 as a mitotic regulator reveals plant-like properties in a non-green lineage. BMC Plant Biol. 15. dx.doi.org/10.1186/s12870-015-0469-6
In: BMC Plant Biology. BioMed Central: London. ISSN 1471-2229; e-ISSN 1471-2229, meer
|   |
| Trefwoorden |
Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]
Marien/Kust |
| Author keywords |
Cyclin-dependent kinase; CDKA2; Cell cycle; Cell division; Cytokinesis;Diatom; Mitosis; Phaeodactylum tricornutum |
| Auteurs | | Top |
- Huysman, M.J.J., meer
- Tanaka, A.
- Bowler, C.
|
|
|
| Abstract |
BackgroundCyclin-dependent kinases (CDKs) are crucial regulators of cell cycle progression in eukaryotes. The diatom CDKA2 was originally assigned to the classical A-type CDKs, but its cell cycle phase-specific transcription at the G2-to-M phase transition is typical for plant-specific B-type CDKs.ResultsHere, we report the functional characterization of CDKA2 from the diatom Phaeodactylum tricornutum. Through a yeast two-hybrid library screen, CDKA2 was found to interact with the G2/M-specific CDK scaffolding factor CKS1. Localization of CDKA2 was found to be nuclear in interphase cells, while in cells undergoing cytokinesis, the signal extended to the cell division plane. In addition, overexpression of CDKA2 induced an overall reduction in the cell growth rate. Expression analysis of cell cycle marker genes in the overexpression lines indicates that this growth reduction is primarily due to a prolongation of the mitotic phase.ConclusionsOur study indicates a role for CDKA2 during cell division in diatoms. The functional characterization of a CDK with clear CDKB properties in a non-green organism questions whether the current definition of B-type CDKs being plant-specific might need revision. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
