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| one publication added to basket [257082] |
Differential transcriptome analysis of the common shrimp Crangon crangon: special focus on the nuclear receptors and RNAi-related genes
Christiaens, O.; Delbare, D.; Van Neste, C.; Cappelle, K.; Yu, N.; De Wilde, R.; Van Nieuwerburgh, F.; Deforce, D.; Cooreman, K.; Smagghe, G. (2015). Differential transcriptome analysis of the common shrimp Crangon crangon: special focus on the nuclear receptors and RNAi-related genes. Gen. Comp. Endocrinol. 212: 163-177. https://dx.doi.org/10.1016/j.ygcen.2014.06.016
In: General and Comparative Endocrinology. Elsevier: New York,. ISSN 0016-6480; e-ISSN 1095-6840, meer
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| Trefwoorden |
Crangon crangon (Linnaeus, 1758) [WoRMS]; Crustacea [WoRMS]; Decapoda [WoRMS]
Marien/Kust |
| Author keywords |
Crangon crangon; Decapoda; Crustacea; Transcriptomics; Nuclearreceptors; RNAi-related genes |
| Auteurs | | Top |
- Christiaens, O., meer
- Delbare, D., meer
- Van Neste, C.
- Cappelle, K., meer
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- Yu, N., meer
- De Wilde, R., meer
- Van Nieuwerburgh, F.
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- Deforce, D.
- Cooreman, K., meer
- Smagghe, G., meer
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| Abstract |
The decapod Crangon crangon is one of the most valuable European fisheries commodities. Despite its economic importance, little sequence data is available for this shrimp species. In this paper, we report the transcriptome sequencing for five different stages of C. crangon (early embryo, late embryo, larva, female adults and male adults) and the annotation and stage-specific expression analysis of nuclear receptors (NRs) and RNA interference (RNAi)-related genes. The NRs are transcription factors that play an essential role in growth, development, cell differentiation, molting/metamorphosis and reproduction, while the RNAi-related genes are very important for internal gene expression regulation and in antiviral defense. We discovered a NR in the female C. crangon which is either a very rapidly evolved homolog of HR10, or a novel NR altogether. This new NR could act as a biological marker for sex determination as it is not expressed in male adults. Most RNAi-related genes were present in C. crangon, proving that the requirements for successful RNAi is present in this decapod shrimp. RNAi-based applications in Crangon such as its use in functional genomics or as antiviral therapeutics could become very important in the near future. |
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