Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [280807] |
Flatworm stem cells and the germ line: developmental and evolutionary implications of macvasa expression in Macrostomum lignano
Pfister, D.; De Mulder, K.; Hartenstein, V.; Kuales, G.; Borgonie, G.; Marx, F.; Morris, J.; Ladurner, P. (2008). Flatworm stem cells and the germ line: developmental and evolutionary implications of macvasa expression in Macrostomum lignano. Dev. Biol. 319(1): 146-159. https://dx.doi.org/10.1016/j.ydbio.2008.02.045
In: Developmental biology. Academic Press: San Diego etc.. ISSN 0012-1606; e-ISSN 1095-564X, meer
|  |
| Trefwoord |
|
| Author keywords |
germline; evolution; neoblasts; planaria; totipotent; vasa |
| Auteurs | | Top |
- Pfister, D.
- De Mulder, K., meer
- Hartenstein, V.
- Kuales, G.
|
- Borgonie, G., meer
- Marx, F.
- Morris, J.
- Ladurner, P.
|
|
| Abstract |
We have isolated and identified the vasa homologue macvasa, expressed in testes, ovaries, eggs and somatic stem cells of the flatworm Macrostomum lignano. Molecular tools such as in situ hybridization and RNA interference were developed for M. lignano to study gene expression and function. Macvasa expression was followed during postembryonic development, regeneration and in starvation experiments. We were able to follow gonad formation in juveniles and the reformation of gonads from stem cells after amputation by in situ hybridization and a specific Macvasa antibody. Expression of macvasa in the germ cells was highly affected by feeding conditions and correlated with the decrease and regrowth of the gonads. RNA interference showed specific down-regulation of macvasa mRNA and protein. The absence of Macvasa did not influence gonad formation and stem cell proliferation. Our results corroborate the exclusive nature of the flatworm stem cell system but challenge the concept of a solely postembryonic specification of the germ line in Platyhelminthes. We address the transition of somatic stem cells to germ cells and speculate on Macrostomum as a system to unravel the mechanisms of preformation or epigenesis in the evolution of germ line specification from somatic stem cells. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
