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Transient ligand docking sites in Cerebratulus lacteus mini-hemoglobin
Deng, P.; Nienhaus, K.; Palladino, P.; Olson, J.S.; Blouin, G.; Moens, L.; Dewilde, S.; Geuens, E.; Nienhaus, G.U. (2007). Transient ligand docking sites in Cerebratulus lacteus mini-hemoglobin. Gene 398(1-2): 208-223. https://dx.doi.org/10.1016/j.gene.2007.01.037
In: Gene. ELSEVIER SCIENCE BV: Tokyo; Oxford; New York; Lausanne; Shannon; Amsterdam. ISSN 0378-1119; e-ISSN 1879-0038, meer
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| Trefwoord |
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| Author keywords |
Fourier Transform Infrared Spectroscopy; photolysis; carbon monoxide;ligand migration |
| Auteurs | | Top |
- Deng, P.
- Nienhaus, K.
- Palladino, P.
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- Olson, J.S.
- Blouin, G.
- Moens, L.
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- Dewilde, S., meer
- Geuens, E.
- Nienhaus, G.U.
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| Abstract |
The monomeric hemoglobin of the nemertean worm Cerebratulus lacteus functions as an oxygen storage protein to maintain neural activity under hypoxic conditions. It shares a large, apolar matrix tunnel with other small hemoglobins, which has been implicated as a potential ligand migration pathway. Here we explore ligand migration and binding within the distal heme pocket, to which the tunnel provides access to ligands from the outside. FTIR/TDS experiments performed at cryogenic temperatures reveal the presence of three transient ligand docking sites within the distal pocket, the primary docking site B on top of pyrrole C and secondary sites C and D. Site C is assigned to a cavity adjacent to the distal portion of the heme pocket, surrounded by the B and E helices. It has an opening to the apolar tunnel and is expected to be on the pathway for ligand entry and exit, whereas site D, circumscribed by TyrB10, GlnE7, and the CD corner, most likely is located on a side pathway of ligand migration. Flash photolysis experiments at ambient temperatures indicate that the rate-limiting step for ligand binding to CerHb is migration through the apolar channel to site C. Movement from C to B and iron-ligand bond formation involve low energy barriers and thus are very rapid processes in the wt protein. |
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