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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
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| one publication added to basket [281557] |
Sequence basis of barnacle cement nanostructure is defined by proteins with silk homology
Barnes, C.R.; Fears, K.P.; Leary, D.H.; Scancella, J.M.; Wang, Z.; Liu, J.L.; Orihuela, B.; Rittschof, D.; Spillmann, C.M.; Wahl, K.J. (2016). Sequence basis of barnacle cement nanostructure is defined by proteins with silk homology. NPG Scientific Reports 6(36219): 14 pp. http://dx.doi.org/10.1038/srep36219
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322, meer
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| Auteurs | | Top |
- Barnes, C.R.
- Fears, K.P.
- Leary, D.H.
- Scancella, J.M.
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- Wang, Z., meer
- Liu, J.L.
- Orihuela, B.
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- Rittschof, D.
- Spillmann, C.M.
- Wahl, K.J.
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| Abstract |
Barnacles adhere by producing a mixture of cement proteins (CPs) that organize into a permanently bonded layer displayed as nanoscale fibers. These cement proteins share no homology with any other marine adhesives, and a common sequence-basis that defines how nanostructures function as adhesives remains undiscovered. Here we demonstrate that a significant unidentified portion of acorn barnacle cement is comprised of low complexity proteins; they are organized into repetitive sequence blocks and found to maintain homology to silk motifs. Proteomic analysis of aggregate bands from PAGE gels reveal an abundance of Gly/Ala/Ser/Thr repeats exemplified by a prominent, previously unidentified, 43 kDa protein in the solubilized adhesive. Low complexity regions found throughout the cement proteome, as well as multiple lysyl oxidases and peroxidases, establish homology with silk-associated materials such as fibroin, silk gum sericin, and pyriform spidroins from spider silk. Distinct primary structures defined by homologous domains shed light on how barnacles use low complexity in nanofibers to enable adhesion, and serves as a starting point for unraveling the molecular architecture of a robust and unique class of adhesive nanostructures. |
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