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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P. (1991). Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish. Antimicrob. Agents Chemother. 35(12): 2486-2489. https://dx.doi.org/10.1128/AAC.35.12.2486 In: Antimicrobial agents and chemotherapy. American Society for Microbiology: Washington, D.C.. ISSN 0066-4804; e-ISSN 1098-6596, meer

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Beschikbaar in	Auteurs
VLIZ: Open access 262329 [ download pdf ]

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Trefwoorden

Aquaculture techniques Cultures > Fish culture Diseases > Infectious diseases Health and safety > Medicine Artemia Leach, 1819 [WoRMS] Brak water; Zoet water

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Auteurs		Top
Nelis, H. Léger, P. Sorgeloos, P., meer De Leenheer, A.P.

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Abstract

The brine shrimp Artemia , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in Artemia franciscana, a sensitive and specific analytical method was developed. It is based on homogenization of Artemia nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C18 cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in A. franciscana, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.

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