Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [289176] |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex
Balzano, S.; Percopo, I.; Siano, R.; Gourvil, P.; Chanoine, M.; Marie, D.; Vaulot, D.; Sarno, D. (2017). Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex. J. Phycol. 53(1): 161-187. https://dx.doi.org/10.1111/jpy.12489
In: Journal of Phycology. Blackwell Science: New York. ISSN 0022-3646; e-ISSN 1529-8817, meer
|   |
| Trefwoorden |
Exploitable Scientific Result
Marine Sciences
Marine Sciences > Marine Genomics
Scientific Community
Scientific Publication
Marien/Kust |
| Author keywords |
biogeography; ITS; ITS2 secondary structure; LSU; morphology; phylogeny;polar diatoms; SSU |
| Project | Top | Auteurs |
- Association of European marine biological laboratories, meer
|
| Auteurs | | Top |
- Balzano, S.
- Percopo, I.
- Siano, R.
- Gourvil, P.
|
- Chanoine, M.
- Marie, D.
- Vaulot, D.
- Sarno, D., meer
|
|
| Abstract |
Seventy-five diatom strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM), as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, and Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA, ITS-1 and ITS-2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi-compensatory base changes (HCBCs) in paired positions of the ITS-2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co-occur in summer phytoplankton communities in the Beaufort Sea. C. neogracilis generally occurred as single cells but also formed short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis, but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
