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| one publication added to basket [289307] |
Genetic delineation between and within the widespread coccolithophore morpho-species Emiliania huxleyi and Gephyrocapsa oceanica (Haptophyta)
Bendif, E.M.; Probert, I.; Carmichael, M.; Romac, S.; Hagino, K.; de Vargas, C. (2014). Genetic delineation between and within the widespread coccolithophore morpho-species Emiliania huxleyi and Gephyrocapsa oceanica (Haptophyta). J. Phycol. 50(1): 140-148. https://dx.doi.org/10.1111/jpy.12147
In: Journal of Phycology. Blackwell Science: New York. ISSN 0022-3646; e-ISSN 1529-8817, meer
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| Trefwoorden |
Climate Change
Environmental Management > Monitoring & Surveillance
Exploitable Scientific Result
Marine Sciences
Marine Sciences > Marine Genomics
Scientific Community
Scientific Publication
Marien/Kust |
| Author keywords |
coccolithophore; DNA barcoding; Emiliania huxleyi; Gephyrocapsaoceanica; phylogeny; species complex |
| Project | Top | Auteurs |
- Association of European marine biological laboratories, meer
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| Auteurs | | Top |
- Bendif, E.M.
- Probert, I.
- Carmichael, M.
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- Romac, S.
- Hagino, K.
- de Vargas, C.
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| Abstract |
Emiliania huxleyi and Gephyrocapsa oceanica are abundant coccolithophore morpho-species that play key roles in ocean carbon cycling due to their importance as both primary producers and cal-cifiers. Global change processes such as ocean acidification impact these key calcifying species. The physiology of E. huxleyi, a developing model species, has been widely studied, but its genetic delineation from G. oceanica remains unclear due to a lack of resolution in classical genetic markers. Using nuclear (18S rDNA and 28S rDNA), mitochondrial (cox1, cox2, cox3, rpl16, and dam), and plastidial (16S rDNA, rbcL, tufA, and petA) DNA markers from 99 E. huxleyi and 44 G. oceanica strains, we conducted a multigene/multistrain survey to compare the suitability of different markers for resolving phylogenetic patterns within and between these two morpho-species. The nuclear genes tested did not provide sufficient resolution to discriminate between the two morpho-species that diverged only 291Kya. Typical patterns of incomplete lineage sorting were generated in phylogenetic analyses using plastidial genes. In contrast, full morpho-species delineation was achieved with mitochondrial markers and common intra-morpho-species phylogenetic patterns were observed despite differing rates of DNA substitution. Mitochondrial genes are thus promising barcodes for distinguishing these coccolithophore morpho-species, in particular in the context of environmental monitoring. |
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