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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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one publication added to basket [289364]
Cryopreservation of the model alga Ectocarpus (Phaeophyceae) Heesch, S.; Day, J.G.; Yamagishi, T.; Kawai, H.; Müller, D.G.; Küpper, F.C. (2012). Cryopreservation of the model alga Ectocarpus (Phaeophyceae). Cryoletters 33(5): 327-336 In: Cryoletters: London. ISSN 0143-2044; e-ISSN 1742-0644, meer

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VLIZ: Non-open access 307257 [ aanvragen ]

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Trefwoorden

Exploitable Scientific Result Fisheries > Gear/Technology Marine Sciences Maritime Industries > Blue Biotech Others Scientific Community Scientific Publication Marien/Kust

Author keywords

Barcoding; brown algae; cryopreservation; Ectocarpus; model organism

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Association of European marine biological laboratories, meer

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Heesch, S. Day, J.G. Yamagishi, T.	Kawai, H. Müller, D.G. Küpper, F.C.

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Abstract

The brown alga Ectocarpus has recently become the first fully sequenced multicellular alga and is an important biological model. Due to the large and growing number of Ectocarpus strains isolated and maintained by the research community, including increasing numbers of mutants, there is an urgent need for developing reliable, cost-effective long-term maintenance techniques. We report here that cryopreservation constitutes an attractive option in this respect, using a simple two-step protocol employing combined DMSO 10% (v/v) and sorbitol 9% (w/v) as cryoprotectants. This model organism appears to be remarkably robust and post-cryo recovery has been observed in all strains tested in this study. Cultures can be regenerated by the germination of cryopreserved zooids (spores), or the recovery of vegetative cells. In the latter case, dividing surviving cells may grow into the cell lumen of a neighbouring dead cell, eventually regenerating a phenotypically normal thalloidal structure.

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