Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [302246] |
Complex evolutionary history of translation elongation factor 2 and diphthamide biosynthesis in archaea and parabasalids
Narrowe, A.B.; Spang, A.; Stairs, C.W.; Caceres, E.F.; Baker, B.J.; Miller, C.S.; Ettema, T.J.G. (2018). Complex evolutionary history of translation elongation factor 2 and diphthamide biosynthesis in archaea and parabasalids. Genome Biology and Evolution 10(9): 2380-2393. https://doi.org/10.1093/gbe/evy154
In: Genome Biology and Evolution. Oxford University Press: Oxford. ISSN 1759-6653; e-ISSN 1759-6653, meer
|   |
| Trefwoorden |
Korarchaeota [WoRMS]; Trichomonas Donné, 1836 [WoRMS]
|
| Author keywords |
Asgard, Korarchaeota, Trichomonas, metagenomics, EF-2, diphthamide. |
| Auteurs | | Top |
- Narrowe, A.B.
- Spang, A., meer
- Stairs, C.W.
- Caceres, E.F.
|
- Baker, B.J.
- Miller, C.S.
- Ettema, T.J.G.
|
|
| Abstract |
Diphthamide is a modified histidine residue which is uniquely present in archaeal and eukaryotic elongation factor 2 (EF-2), an essential GTPase responsible for catalyzing the coordinated translocation of tRNA and mRNA through the ribosome. In part due to the role of diphthamide in maintaining translational fidelity, it was previously assumed that diphthamide biosynthesis genes (dph) are conserved across all eukaryotes and archaea. Here, comparative analysis of new and existing genomes reveals that some archaea (i.e., members of the Asgard superphylum, Geoarchaea, and Korarchaeota) and eukaryotes (i.e., parabasalids) lack dph. In addition, while EF-2 was thought to exist as a single copy in archaea, many of these dph. lacking archaeal genomes encode a second EF-2 paralog missing key residues required for diphthamide modification and for normal translocase function, perhaps suggesting functional divergence linked to loss of diphthamide biosynthesis. Interestingly, some Heimdallarchaeota previously suggested to be most closely related to the eukaryotic ancestor maintain dph. genes and a single gene encoding canonical EF-2. Our findings reveal that the ability to produce diphthamide, once thought to be a universal feature in archaea and eukaryotes, has been lost multiple times during evolution, and suggest that anticipated compensatory mechanisms evolved independently. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
