Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [307904] |
Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection
Yaacob, E.N.; De Geest, B.G.; Goethals, J.; Bajek, A.; Dierckens, K.; Bossier, P.; Vanrompay, D. (2018). Recombinant ferritin-H induces immunosuppression in European sea bass larvae (Dicentrarchus labrax) rather than immunostimulation and protection against a Vibrio anguillarum infection. Vet. Immunol. Immunopathol. 204: 19-27. https://dx.doi.org/10.1016/j.vetimm.2018.09.001
In: Veterinary Immunology and Immunopathology. Elsevier: Tokyo; Oxford; Lausanne; New York; Shannon; Amsterdam. ISSN 0165-2427; e-ISSN 1873-2534, meer
|  |
| Trefwoorden |
Dicentrarchus labrax (Linnaeus, 1758) [WoRMS]; Listonella anguillara (Bergeman, 1909) MacDonell & Colwell, 1985 [WoRMS]
|
| Author keywords |
Sea bass; Larvae; Gnotobiotic; Vibrio anguillarum; Ferritin; Innateimmunity |
| Auteurs | | Top |
- Yaacob, E.N., meer
- De Geest, B.G., meer
- Goethals, J., meer
- Bajek, A.
|
|
|
| Abstract |
Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture. In this study, we evaluated if the recombinant sea bass ferritin-H could stimulate the innate immune system of gnotobiotic European sea bass larvae resulting in protection against a V. anguillarum challenge. We also evaluated the effect of a V. anguillarum infection on the transcription of immune-related genes in gnotobiotic European sea bass larvae. Recombinant sea bass ferritin-H was produced, encapsulated in calcium alginate microparticles and orally delivered to sea bass larvae at seven days after hatching. Our results showed V. anguillarum caused an acute infection, resulting in high mortality. The infection significantly upregulated the expression of tlr3, tlr5, cas1, il1β, tnfα, mif, il10, cc1, cxcl8 at 18, 24 and 36 h post infection, but not of the chemokine receptor genes cxcr4 and ccr9. There was no protective effect of ferritin-H. Remarkably, ferritin-H caused significantly higher transcript levels for cxcr4 and ccr9. Sea bass ferritin-H was more likely involved in immune-suppression and results point in the direction of a negative regulation of CXCR4 resulting in inhibition of cell proliferation, differentiation and migration which is detrimental to innate immunity and might explain the non-protective effect of ferritin-H in fish larvae. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
