Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [3282] |
Measurement of the adenylate energy charge in Nereis diversicolor and Nephtys sp. (Polychaeta: Annelida): evaluation of the usefulness of AEC in pollution monitoring
Verschraegen, K.; Herman, P.M.J.; Van Gansbeke, D.; Braeckman, A. (1985). Measurement of the adenylate energy charge in Nereis diversicolor and Nephtys sp. (Polychaeta: Annelida): evaluation of the usefulness of AEC in pollution monitoring. Mar. Biol. (Berl.) 86: 233-240
In: Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793, meer
|  |
| Trefwoorden |
Chemical compounds > Phosphorus compounds > Phosphates > Nucleotides > AMP
Chemical compounds > Phosphorus compounds > Phosphates > Nucleotides > ATP
Efficiency
Monitoring > Environmental monitoring > Pollution monitoring
Annelida [WoRMS]; Nephtys Cuvier, 1817 [WoRMS]; Nereis diversicolor Müller, 1776 [WoRMS]
België, Zeeschelde [Marine Regions]
Marien/Kust |
| Auteurs | | Top |
- Verschraegen, K.
- Herman, P.M.J., meer
- Van Gansbeke, D., meer
- Braeckman, A.
|
|
|
| Abstract |
ATP-content and adenylate energy charge (AEC) ratios were determined in two polychaete species (Nereis diversicolor and Nephtys sp.), sampled in ten stations along the heavily polluted Western Scheldt estuary (N. Belgium, S. Holland). The samples were taken between 27 December 1982 and 6 January 1983. Nereis diversicolor was also sampled in an unpolluted brackish water pond, and subjected to artificial stress by drying the organisms on filter paper. Adenine nucleotide levels were determined using the firefly bioluminescence reaction. The light emission was measured with the integration mode. As the "Constant Light Signal" reagent used contained APM and probably ADP itself, both myokinase and pyruvatekinase had to be inactivated before the assay. The use of pepsin in acidic conditions to denaturate these enzymes is discussed. For both N. diversicolor and Nephtys sp., a significant relation between ATP-content and fresh weight is found in most samples. Within each species, ATP-content per g fresh weight is dependent on the sampling method, but is not significantly different between stations. It is higher in Nephtys sp. from the W. Scheldt (130.8 10-5 g ATP/g fresh weight) than in N. diversicolor from the Dievengat (83.9 10-5g ATP/g fresh weight). This value in turn is significantly higher than that for N. diversicolor in the W. Scheldt (64.0 10-5 g ATP/g fresh weight). In the drying experiment, AEC gradually decreases with increasing drying time. In the field, AEC is generally high and not significantly different between the three populations. AEC ratios also do not differ between stations within each population. It is argued that field monitoring of AEC in invertebrates is not valuable as a pollution assessment tool for two reasons. In polluted stations, only pollutant-resistant species will be found, and there is evidence that the maintenance of a stable population is impossible when the individuals constantly have low AECs. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
