Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [337862] |
Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana
Iryani, M.T.M.; Sorgeloos, P.; Danish-Daniel, M.; Tan, M.P.; Wong, L.L.; Mok, W.J.; Satyantini, W.H.; Mahasri, G.; Sung, Y.Y. (2020). Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana. Cell Stress Chap. 25(6): 1099-1103. https://hdl.handle.net/10.1007/s12192-020-01113-0
In: Cell Stress and Chaperones. Springer: New York. ISSN 1355-8145; e-ISSN 1466-1268, meer
|  |
| Trefwoord |
Artemia franciscana Kellog, 1906 [WoRMS]
|
| Author keywords |
Hsp70; Artemia franciscana; Cysts; RNA interference (RNAi) |
| Auteurs | | Top |
- Iryani, M.T.M.
- Sorgeloos, P., meer
- Danish-Daniel, M.
|
- Tan, M.P.
- Wong, L.L.
- Mok, W.J.
|
- Satyantini, W.H.
- Mahasri, G.
- Sung, Y.Y., meer
|
| Abstract |
Females of the brine shrimp Artemia franciscana produce either free-swimming nauplii via ovoviviparous pathway of reproduction or encysted embryos, known as cysts, via oviparous pathway, in which biological processes are arrested. While previous study has shown a crucial role of ATP-dependent molecular chaperone, heat shock protein 70 (Hsp70) in protecting A. franciscana nauplii against various abiotic and abiotic stressors, the function of this protein in diapausing embryos and cyst development, however, remains unknown. RNA interference (RNAi) was applied in this study to examine the role of Hsp70 in cyst development and stress tolerance, with the latter performed by desiccation and freezing, a common method used for diapause termination in Artemia cysts. Hsp70 knockdown was apparent in cysts released from females that were injected with Hsp70 dsRNA. The loss of Hsp70 affected neither the development nor morphology of the cysts. The time between fertilization and cyst release from Artemia females injected with Hsp70 dsRNA was delayed slightly, but the differences were not significant when compared to the controls. However, the hatching percentage of cysts which lacks Hsp70 were reduced following desiccation and freezing. Taken together, these results indicated that Hsp70 possibly plays a role in the stress tolerance but not in the development of diapause-destined embryos of Artemia. This research makes fundamental contributions to our understanding of the role molecular chaperone Hsp70 plays in Artemia, an excellent model organism for diapause studies of the crustaceans. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
