Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
one publication added to basket [355934] |
Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from ‘mam nem’, a fermented fish sauce
Thuy, D.T.B.; Nguyen, A.T.; Khoo, K.S.; Chew, K.W.; Cnockaert, M.; Vandamme, P.; Ho, Y.-C.; Huy, N.D.; Cocoletzi, H.H.; Show, P.L. (2020). Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from ‘mam nem’, a fermented fish sauce. Bioengineered 12(1): 54-62. https://dx.doi.org/10.1080/21655979.2020.1857626
In: Bioengineered. TAYLOR & FRANCIS INC: Philadelphia. ISSN 2165-5979; e-ISSN 2165-5987, meer
| |
Trefwoorden |
Prokaryotes > Microorganisms > Bacteria > Firmicutes > Streptococcaceae > Pediococcus > Pediococcus pentosaceus Marien/Kust |
Author keywords |
GABA; optimization; Pediococcus pentosaceus; fermented fish sauce |
Auteurs | | Top |
- Thuy, D.T.B.
- Nguyen, A.T.
- Khoo, K.S.
- Chew, K.W.
|
- Cnockaert, M., meer
- Vandamme, P., meer
- Ho, Y.-C.
|
- Huy, N.D.
- Cocoletzi, H.H.
- Show, P.L.
|
Abstract |
This study was aimed to identify and optimize the culture conditions for gamma-aminobutyric acid (GABA) production by a lactic acid bacterium strain isolated from mam nem, a fermented fish sauce. Among the six isolates obtained from mam nem, the MN12 had the most potent GABA-producing capability. The strain was then identified to be Pedioccocus pentosaceus by employing MALDI-TOF-MS and phenylalanyl-tRNA synthase sequencing methods. The initial cell density of 5.106 CFU/mL, monosodium glutamate concentration of 60 mM, initial pH of 7, temperature of 45°C and cultivation time of 72 h were found to be the optimal culture conditions for highest production of GABA, reaching 27.9 ± 0.42 mM, by this strain. The cultivation conditions for GABA production by P. pentosaceus MN12 have been successfully optimized, providing a foundation for the development of fermented foods enriched with GABA. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.