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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
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Effects of field metal contamination on sperm motility and RNA/DNA ratio in two echinoderm species = Efeito da acumulação de metais pesados na motilidade do esperma e no índice ARN/ADN de populações de duas espécies de equinodermes
dos Ramos Catarino, A.I. (2006). Effects of field metal contamination on sperm motility and RNA/DNA ratio in two echinoderm species = Efeito da acumulação de metais pesados na motilidade do esperma e no índice ARN/ADN de populações de duas espécies de equinodermes. MA Thesis. Universidade de Évora: Lisboa. 13 pp. https://hdl.handle.net/10174/16151
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Documenttype: Doctoraat/Thesis/Eindwerk
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- dos Ramos Catarino, A.I., meer
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| Abstract |
This study aimed to assess the effects of field metal contamination on sperm motility and RNA/DNA ratio in two populations of echinoderms; Asterias rubens and Echinus acutus. These species occur naturally along a contamination gradient of cadmium, copper, lead and zinc, in a Norwegian Fjord (Sørfjord). Sperm motility was quantified with the help of a computer assisted sperm analysis system (CASA). The RNA/DNA ratios were assessed based on a l-dye, (Ethidium bromide)/l-enzyme (RNase) 96-well microplate fluorometric assay. Although both species appeared to readily accumulate metals, neither sperm motility parameters in A. rubens nor RNA/DNA in the body wall of both species were affected. The RNA/DNA ratios in A. rubens pyloric caeca were significantly higher in the most contaminated station. Even thought this fjord is considered a highly contaminated place, these populations seem to tolerate these metal contamination levels. |
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