Catalogus

Zoeken

Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.

Boekenmand

Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).

RSS

Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.

log in

Publicaties | Kaarten

nieuwe zoekopdracht

[ meld een fout in dit record ]

mandje (1): toevoegen | toon

---

one publication added to basket [210049]

Microsatellite DNA marker development and genetic diversity of Branchiostoma belcheri in Xiamen waters Liu, W.-y.; Zhong, J.; Xu, W.; Wang, Y.-Q. (2011). Microsatellite DNA marker development and genetic diversity of Branchiostoma belcheri in Xiamen waters. Mar. Biol. Res. 7(8): 826-861. https://dx.doi.org/10.1080/17451000.2011.569553 In: Marine Biology Research. Taylor & Francis: Oslo; Basingstoke. ISSN 1745-1000; e-ISSN 1745-1019

---

Beschikbaar in	Auteurs
VLIZ [ aanvragen ]

---

Trefwoorden

Acids > Organic compounds > Organic acids > Nucleic acids > DNA Genetic diversity Microsatellites Branchiostoma belcheri tsingtauense Tchang-Shi & Koo, 1936 [WoRMS] Marien/Kust

Author keywords

Amphioxus; Branchiostoma belcheri; DNA molecular marker; geneticdiversity; microsatellite DNA

---

Auteurs		Top
Liu, W.-y. Zhong, J. Xu, W. Wang, Y.-Q.

---

Abstract

Amphioxus occupies a transitional position between invertebrates and vertebrates and is therefore a promising laboratory model animal. As a powerful molecular marker in population genetics studies, microsatellite DNA is required for both the investigation of genetic diversity of a wild amphioxus population and strain breeding of laboratory cultured amphioxus. In the present study, we constructed a microsatellite-enriched DNA library of amphioxus Branchiostoma belcheri to develop this molecular marker. After screening the library and combining it with the data obtained from a public database, we successfully developed 21 pair of primers that could effectively amplify the microsatellite loci of amphioxus. Of those primer pairs, seven were in deviation of Hardy–Weinberg equilibrium, mostly caused by null alleles. The others had no linkage disequilibrium, indicating they are applicable for the genetic studies. Using those primer pairs, we further detected the genetic diversity of the wild amphioxus population in Xiamen waters. The results revealed that the number of alleles of each microsatellite locus was between three and 18 in the population, and observed and expected heterozygosities of the loci ranged from 0.415 to 0.947 and from 0.300 to 1.000, indicating very high genetic diversity of the population.

---

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid

Top | Auteurs