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| one publication added to basket [239354] |
Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei
Phuoc, L.H.; Corteel, M.; Thanh, N.C.; Nauwynck, H.J.; Pensaert, M.B.; Alday-Sanz, V.; Van Den Broeck, W.; Sorgeloos, P.; Bossier, P. (2009). Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei. Aquaculture 290(1-2): 61-68. dx.doi.org/10.1016/j.aquaculture.2009.02.004
In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622
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| Trefwoorden |
Penaeus vannamei Boone, 1931 [WoRMS]; Vibrio Pacini, 1854 [WoRMS]
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| Author keywords |
Co-infection; Challenge route; Dose; Shrimp; Vibrio; WSSV |
| Auteurs | | Top |
- Phuoc, L.H.
- Corteel, M.
- Thanh, N.C.
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- Nauwynck, H.J.
- Pensaert, M.B.
- Alday-Sanz, V.
|
- Van Den Broeck, W.
- Sorgeloos, P.
- Bossier, P.
|
| Abstract |
This study was conducted to investigate the effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Penaeus vannamei shrimp. Juvenile shrimp were first injected with WSSV at a dose of 30 SID50 shrimp- 1 (SID50 = shrimp infectious dose with 50% endpoint) and 24 h later with 103, 104, 105 or 106 CFU shrimp- 1 of V. campbellii. Controls did not die during the experiment, except the ones that received 106 CFU shrimp- 1 (35–65%). In WSSV-inoculated shrimp, the 100% cumulative mortality were reached at 144–360 h post injection (hpi). WSSV-infected shrimp died much faster when injected with at least 104 CFU of V. campbellii with the 100% cumulative mortality reached at 48–96 hpi of virus. The density of V. campbellii in haemolymph of co-infected moribund shrimp collected 6 h after V. campbellii injection was significantly higher than that in shrimp injected with V. campbellii only. There was no difference in the number of WSSV-infected cells between shrimp inoculated with WSSV only, compared to dually inoculated ones. Shrimp which were first injected with WSSV and 24 h (or 48 h) later exposed to 106, 107, or 108 CFU ml- 1 of V. campbellii by immersion did not show any accelerated mortality. When WSSV-infected shrimp were challenged with another Vibrio species, V. harveyi BB120, no accelerated mortality was noted in WSSV-infected shrimp injected with 106 CFU shrimp- 1 of V. harveyi BB120.In conclusion, it can be stated that the synergistic effect between WSSV and Vibrio is influenced by the dose, species and infection route of inoculation of the Vibrio bacteria. |
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