Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
| one publication added to basket [247029] |
Protein degradation during the diatom cell cycle: annotation and transcriptional analysis of SCF and APC/C ubiquitin ligase genes in Phaeodactylum tricornutum
Huysman, M.J.J.; Martens, C.; Vyverman, W.; De Veylder, L. (2014). Protein degradation during the diatom cell cycle: annotation and transcriptional analysis of SCF and APC/C ubiquitin ligase genes in Phaeodactylum tricornutum. Marine Genomics 14: 39-46. dx.doi.org/10.1016/j.margen.2013.09.001
In: Marine Genomics. Elsevier: Amsterdam. ISSN 1874-7787; e-ISSN 1876-7478
|  |
| Trefwoorden |
Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]
Marien/Kust |
| Author keywords |
Proteolysis; Cell cycle; Proteasome; CDC20; CDH1 |
| Auteurs | | Top |
- Huysman, M.J.J.
- Martens, C.
- Vyverman, W.
- De Veylder, L.
|
|
|
| Abstract |
In every eukaryotic organism, unidirectional cell cycle progression is driven by controlled proteolysis. Here, we present the identification of two ubiquitin ligase complexes in the diatom Phaeodactylum tricornutum, the SCF and APC/C, being important for temporal controlled degradation of key cell division proteins. We annotated and analyzed the conservation of all subunits of both complexes in P. tricornutum. Expression analysis during a synchronized cell cycle showed that the SCF complex subunits are transcribed at the G1-to-S phase transition. In contrast, expression of the APC/C subunits is relatively constant, except for its activators that are differentially expressed: CDC20 is highly expressed at mitosis, while CDH1 is transcribed at late M and during G1, suggesting temporal activation of the different complexes. Furthermore, we performed in silico prediction of APC/C targets through destruction box (D-box) and KEN box analysis, two known degrons for substrate recognition of the APC/C complexes. For this, we focused on the expanded set of diatom cyclins, including the diatom-specific cyclins. Interestingly, we could find D-boxes for most mitotically expressed cyclins, but also some of the G1/S cyclins. Thus, it appears that in analogy with what is known in other organisms, tight post-translational control of the diatom cyclins might contribute to the well-coordinated cell cycle progression. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
